Both baseline and most recent lipoproteinassociated. Evaluation of lipoproteinassociated phospholipase a2 in. Lipoproteinassociated phospholipase a2, or lppla2, is an enzyme that travels alongside lowdensity lipoprotein ldl. Lipoproteinassociated phospholipase a2, creactive protein, and fibrinogen levels, and the whitecell count were measured at base line, along with other traditional risk factors. Lipoproteinassociated phospholipase a2 lp pla2 antibody. Lipoproteinassociated phospholipase a2 lppla2 is a calciumindependent phospholipase a2 enzyme secreted by leukocytes and associated with circulating lowdensity lipoprotein and macrophages in atherosclerotic plaques.
The lipoprotein associated phospholipase a2 lppla2plac test is typically used to assess a persons risk for certain types of cardiovascular disease as well as events like a heart attack. Previously, studies have confirmed lipoproteinassociated phospholipase a2 lppla2 as a novel type of inflammatory marker in coronary heart. Lipoproteinassociated phospholipase a2 lppla2 was identified as a strong predictor for cardiovascular events. Siscapa, stable isotope standards and capture by antipeptide antibody. Lipoproteinassociated phospholipase a 2 springerlink. Lipoproteinassociated phospholipase a2 activity in obese. Laertes lipoproteinassociated phospholipase a2 in stable coronary artery disease study, which is an ongoing prospective, hospitalbased study investigating the prognostic impact of lppla 2 in stable cad. Both baseline and most recent lipoprotein associated phospholipase a2 activity levels are associated with coronary artery calcification in type 1 diabetes lipoprotein associated phospho lipoprotein associated phospholipase a2 lppla2 activity has previously been associated with coronary artery calcification cac, however this relationship has not been well studied in type 1 diabetes t1d. This test looks for a specific lipoprotein, lppla2, in your blood. There is a strong association between lipoprotein associated phospholipase a 2 lppla 2 levels and atherosclerosisrelated diseases. Lipoprotein associated phospholipase a2 has been shown to be an independent risk factor for coronary heart disease and ischemic stroke in several studies. In east asians, a lossoffunction variant in the pla2g7 gene can be used to assess the effects of genetically determined lower lppla 2. Lipoprotein associated phospholipase a2 lppla2 as an. Lipoproteinassociated phospholipase a2 an overview.
Custom preparations, technical support, bulk quantities and aliquoting available, email. It is an enzyme produced by macrophages that hydrolyzes phospholipids of oxidized lowdensity lipoprotein ldl, releasing oxidized fatty acids and lysophosphatidylcholine, which are potent proin ammatory and prooxidative molecules. Pafah catalyzes the removal of the acyl group at the sn2 position of paf and truncated phospholipids generated in settings of. Another fragment, 33, was confirmed to bind near the binding pocket of 32, and merging these. Lipoproteinassociated phospholipase a2 in coronary heart. Coronary heart disease, lipoproteinassociated phospholipase a2, darapladib, inflammation, myocardial infarction, atherosclerosis.
The new lipoproteinassociated phospholipase a2 activity, serum, test is now available to the mayo clinic practice and mayo medical laboratories clients. We initially screened 620 patients, of whom 524 satis. Lipoproteinassociated phospholipase a 2 lp pla 2 is an enzyme that appears to play a role in the inflammation of blood vessels and is thought to help promote atherosclerosis. Crosssectional study comparing lppla2 and hscrp as predictors of ms in asymptomatic subjects was carried out. Lipoprotein associated phospholipase a2 lppla2 is an emerging inflammatory marker that is used to assess the risk for cardiovascular disease cvd and associated events.
Accumulating evidence has suggested that lppla2 is a biomarker of cad and may have a proinflammatory role in the progression of atherosclerosis. The elevated lipoproteinassociated phospholipase a2 activit. Mass levels of lipoproteinassociated phospholipase a 2 lppl a 2, a leukocytederived enzyme involved in the metabolism of lowdensity lipoprotein to proinflammatory mediators, are associated with prognosis after stroke. Lipoprotein associated phospholipase a 2 mass was measured with an enzymelinked immunoassay according to previously described methods. Lipoproteinassociated phospholipase a2 bound on highdensity. Lipoproteinassociated phospholipase a2 lppla 2 is a novel biomarker of vascularspecific inflammation that provides information about atherosclerotic plaque inflammation and stability. These findings necessitate the need for more reliable and accurate biomarkers to predict cvd.
Lppla2 hydrolyzes plateletactivating factor paf and oxidizes phospholipids with a modified short fatty acyl chain esterified at the sn2 position. Lipoproteinassociated phospholipase a2 lp pla2 progenitor cells and coronary atherosclerosis in humans the safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Lipoprotein associated phospholipase a2 the journal of lipid. Fragmentbased approach to the development of an orally. A total of 328 hospitalized patients were recruited. Is lipoproteinassociated phospholipase a2 activity correlated with. Lipoproteinassociated phospholipase a2 is associated with angiographic. Patients with high lipoproteinassociated phospholipase a2 concentrations greater than or equal to 235 ngml are at increased risk for cardiovascular events including myocardial infarction and ischemic. Lppla 2 is a calciumindependent phospholipase a2 enzyme that is associated with both lowdensity lipoprotein ldl and, to a lesser extent, highdensity lipoprotein hdl in human plasma and serum 1 and is distinct from other such phospholipases such as cpla 2 and spla 2. The aim of this study was to investigate the role of lppla 2 in the ischemic stroke and further offer clinical evidence that measuring lppla 2 helps predict the risk of stroke occurrence and recurrence. Lipoproteinassociated phospholipase a2 has been shown to be an independent risk factor for coronary heart disease and ischemic stroke in several studies. Previously, studies have confirmed lipoproteinassociated phospholipase a2 lp pla2 as a novel type of inflammatory marker in coronary heart. Lipoproteinassociated phospholipase a2 american journal of. Lppla2 levels in 763 healthy chinese han subjects 392 males and 371 females were determined by colorimetric analysis and the.
This test measures the amount or activity of lppla 2 in the blood. This chapter is focused on the plasma form of pafacetylhydrolase pafah, a lipoproteinbound, calciumindependent phospholipase a 2 activity also referred to as lipoproteinassociated phospholipase a2 and pla2g7. Lipoproteinassociated phospholipase a2 lppla2 antibodies. Lipoproteinassociated phospholipase 2 definition of. The elevated lipoproteinassociated phospholipase a2. Lipoproteinassociated phospholipase a2 request pdf. Jul 28, 2015 the new lipoprotein associated phospholipase a2 activity, serum, test is now available to the mayo clinic practice and mayo medical laboratories clients. Product classification food and drug administration. There is a strong association between lipoproteinassociated phospholipase a 2 lppla 2 levels and atherosclerosisrelated diseases. Lipoproteinassociated phospholipase a2 test request a test. Lipoproteinassociated phospholipase a2 pathophysiological.
The lipoprotein associated phospholipase a2, immunoassay, system, test, is intended to measure lipoprotein associated phospholipase a2 in human plasma in conjunction with clinical evaluation and other patient risk factors including biochemical analyses as an aid in predicting risk for coronary heat disease. Evidence has gathered that inflammation plays a crucial role in the triggering and progression of atherosclerosis. Lipoproteinassociated phospholipase a2 lp pla2 clinical and research area cardiac markers clinical utility prognostic marker of adverse cardiacrelated events 1. Lipoprotein associated phospholipase a2 is a proinflammatory enzyme secreted by macrophages that is primarily bound to ldl in the circulation. Aug 16, 2012 the role of inflammation in atherosclerosis continues to emerge.
Pdf recent developments with lipoproteinassociated. The plac test for lppla 2 activity is an enzyme assay for the in vitro quantitative determination of lppla 2 lipoproteinassociated phospholipase a 2 activity in edta plasma and serum on automated clinical chemistry analyzers. The lipoproteinassociated phospholipase a2, immunoassay, system, test, is intended to measure lipoproteinassociated phospholipase a2 in human plasma in conjunction with clinical evaluation and other patient risk factors including biochemical analyses as an aid in. Patients with high lipoprotein associated phospholipase a2 concentrations greater than or equal to 235 ngml are at increased risk for cardiovascular events including myocardial infarction and ischemic stroke.
Lipoprotein associated phospholipase a2 lppla2 antigen profile protein summary the protein encoded by this gene is a secreted enzyme that catalyzes the degradation of plateletactivating factor to biologically inactive products. Lppla2 is an enzyme specific to arterial inflammation making it a promising biomarker for predicting the development of cvd. Elevated plasma lipoproteinassociated phospholipase a2 activity is associated with plaque rupture in patients with coronary artery disease. Lipoproteinassociated phospholipase a2 activity and risk. Measurement of lipoproteinassociated phospholipase a2 lp. The test is used to help predict your risk for cardiovascular disease and stroke. Lipoproteinassociated phospholipase a2 is associated with.
Lipoproteinassociated phospholipase a2 lppla2 antibody. Lipoprotein associated phospholipase a 2 lppla 2 is an enzyme that appears to play a role in the inflammation of blood vessels and is thought to help promote atherosclerosis. Lipoprotein phospholipase a2 reference lab affiliated. There are no reports comparing these markers as predictors of ms. Background lppla 2 is a novel risk factor for cardiovascular disease. Lipoproteinassociated phospholipase a2 lppla2 progenitor. It is believed to contribute to hardening of the arteries atherosclerosis lipoproteins carry cholesterol and fats through blood. Lipoproteinassociated phospholipase a2 as a predictive. Lipoproteinassociated phospholipase a2 lp pla2, in vitro, antibody cat. Recombinant human lipoproteinassociated phospholipase a2 lppla2 cat. Coronary heart disease, lipoprotein associated phospholipase a2, darapladib, inflammation, myocardial infarction, atherosclerosis.
Sep 26, 2017 lipoprotein associated phospholipase a2, plateletactivating factor acetylhydrolase, generates two bioactive products during the oxidation of lowdensity lipoprotein. Is lipoprotein associated phospholipase a2 activity correlated with. Clinical chemistry, immunology and laboratory quality control, 2014. Several epidemiological studies have shown that circulating levels of lppla2 are an independent risk factor for cardiovascular events. Comparison of lipoproteinassociated phospholipase a2 and. In the last decades, the role of inflammation in the pathogenesis of atherosclerosis has been the topic of intense research. Oct, 2017 lipoprotein associated phospholipase a2 lppla2 was identified as a strong predictor for cardiovascular events.
In both primary and secondary prevention study populations, lppla2 was consistently associated with higher cardiovascular risk, and the risk estimate appears to be relatively unaffected by. Lipoproteinassociated phospholipase a2 lp pla2, also known as plateletactivating factor acetylhydrolase, is an enzyme that hydrolyzes phospholipids and is primarily associated with ldls. Lipoproteinassociated phospholipase a2 lppla2, also known as platelet activating factor. Measure lipoproteinassociated phospholipase a2 lppla 2, which in some studies has demonstrated more specificity than hscrp, when it is necessary to further stratify an individuals ascvd risk, especially in the presence of hscrp elevations grade a. The aim of this study is to establish lipoprotein associated phospholipase a2 lppla2 reference intervals ris in healthy chinese han adults as a clinical diagnostic indicator according to the clinical and laboratory standards institute clsi c28a3 guidelines. Lipoprotein associated phospholipase a2 lppla 2 is a novel biomarker of vascularspecific inflammation that provides information about atherosclerotic plaque inflammation and stability. Both baseline and most recent lipoproteinassociated phospholipase a2 activity levels are associated with coronary artery calcification in type 1 diabetes lipoproteinassociated phospho lipoproteinassociated phospholipase a2 lppla2 activity has previously been associated with coronary artery calcification cac, however this relationship has not been well studied in type 1 diabetes t1d. Lipoprotein associated phospholipase a2 does this test have other names. Lipoprotein associated phospholipase a2 lppla2 also predicts cv disease.
After adding the product to the cart by clicking the button below you will see if there is a volume discount available. Measurement of lipoproteinassociated phospholipase a2 lppla2 in the assessment of cardiovascular risk 2 background lowdensity lipoproteins ldl have been identified as the major atherogenic lipoproteins and have long been identified by the national cholesterol education project ncep as the primary target of cholesterollowering therapy. In the circulation, approximately 80% oflppla 2 is bound to ldl particles, where it hydrolyzes phospholipids within oxidized ldl, thereby leading to the production of the proinflammatory byproducts lysophosphatidylcholineand oxidized fatty acids. Lipoproteinassociated phospholipase a2 health encyclopedia. Lipoproteinassociated phospholipase a2 activity, serum. Lipoproteinassociated phospholipase a 2 mass was measured with an enzymelinked immunoassay according to previously described methods. Elevated plasma lipoprotein associated phospholipase a2 activity is associated with plaque rupture in patients with coronary artery disease. Lipoproteinassociated phospholipase a2 is a proinflammatory enzyme secreted by macrophages that is primarily bound to ldl in the circulation. Lipoproteinassociated phospholipase a2 lppla2, a novel plasma biomarker, circulates in the blood bound mainly to lowdensity lipoprotein ldl and promotes vascular inflammation. Lee biosolutions is the leading supplier of recombinant human lipoprotein associated phospholipase a2 lppla2 antigen for life science, laboratory, research and diagnostic manufacturing uses. Lipoproteinassociated phospholipase a2 lppla2 plac. Therefore, we compared lppla2 activity between two obese age, sex, and bmimatched.
Lppla2 is an enzyme which is usually bound to ldl, often called the bad cholesterol, in the blood and plays a role in the inflammation of blood vessels. Lipoprotein associated phospholipase a 2 lppla 2 also known as plateletactivating factor acetylhydrolase pafah is a phospholipase a 2 enzyme that in humans is encoded by the pla2g7 gene. Lee biosolutions is the leading supplier of recombinant human lipoproteinassociated phospholipase a2 lppla2 antigen for life science, laboratory, research and diagnostic manufacturing uses. Lipoproteinassociated phospholipase a2 does this test have other names. This test measures the amount or activity of lp pla 2 in the blood some recent studies have shown that lp pla 2 is an independent risk marker for cardiovascular disease cvd, including coronary heart disease chd. Lipoproteinassociated phospholipase a2 lppla2 has been hypothesized to be involved in atherogenesis through pathways related to inflammation. Research article comparison of lipoproteinassociated. This study was undertaken to assess the role of lipoprotein associated phospholipase a2 lp pla2 as a biomarker to predict cvd in patients with metabolic syndrome ms among south indian population. The role of inflammation in atherosclerosis continues to emerge. Ballantyne and colleagues 7 studied lppla2 in the 12,762 apparently healthy individuals participating in the aric study. Lipoproteinassociated phospholipase a 2 lppla 2 is an enzyme produced by white blood cells, notably macrophages and lymphocytes. Lipoproteinassociated phospholipase a2 lp pla2 progenitor cells and coronary atherosclerosis in humans the safety and scientific validity of this study is the.
Lipoproteinassociated phospholipase a2 as an independent. Recombinant human lipoproteinassociated phospholipase a2. Lipoproteinassociated phospholipase a 2 lppla 2 also known as plateletactivating factor acetylhydrolase pafah is a phospholipase a 2 enzyme that in humans is encoded by the pla2g7 gene. No matter where you are in your career, we would welcome you and encourage you to consider joining our community. Measurement of lipoproteinassociated phospholipase a2. Lipoproteinassociated phospholipase a2 lppla2, also known as plateletactivating factor. The lipoproteinassociated phospholipase a2 lppla2plac test is typically used to assess a persons risk for certain types of cardiovascular disease as well as events like a heart attack. Mean levels of both lppla2 and creactive protein were. Pdf lipoproteinassociated phospholipase a2 and risk of. Lipoprotein associated phospholipase a2 lppla2, a novel plasma biomarker, circulates in the blood bound mainly to lowdensity lipoprotein ldl and promotes vascular inflammation. Lipoprotein associated phospholipase a2 lppla2 has been hypothesized to be involved in atherogenesis through pathways related to inflammation. This test measures the amount or activity of lppla 2 in the blood some recent studies have shown that lppla 2 is an independent risk marker for cardiovascular disease cvd, including coronary heart disease chd. Lipoproteinassociated phospholipase a2 and its relationship. Measurement of lipoprotein associated phospholipase a2 lppla2 in the assessment of cardiovascular risk 2 background lowdensity lipoproteins ldl have been identified as the major atherogenic lipoproteins and have long been identified by the national cholesterol education project ncep as the primary target of cholesterollowering therapy.
Lipoproteinassociated phospholipase a 2 lppla 2 has been implicated in development of atherosclerosis. Dietary, lifestyle, and clinical predictors of lipoproteinassociated. Pla2 belongs to group vii of the pla2 superfamily, which currently comprises of six types and is divided into 16 unique groups. Lipoproteinassociated phospholipase a2 and coronary heart.
The aim of the present study was to observe the role of lipoproteinassociated phospholipase a2 lppla2 and antithrombin iii atiii in. Elevated levels of serum lppla 2 are indicative of rupture prone plaque and a strong independent predictor of cardiovascular risk, including coronary artery. Evidence has gathered that inflammation plays a crucial. Lactam inhibitor of lipoproteinassociated phospholipase a2 lppla2. Why measure lppla 2 measure lipoproteinassociated phospholipase a2 lppla 2, which in some studies has demonstrated more specificity than hscrp, when it is necessary to further stratify an individuals ascvd risk, especially in the presence of hscrp elevations grade a. May 09, 2019 lipoprotein associated phospholipase a2, or lppla2, is an enzyme that travels alongside lowdensity lipoprotein ldl. Predictive value of combining the level of lipoproteinassociated. Lipoproteinassociated phospholipase a2 bound on high.
Lipoproteinassociated phospholipase a2 lppla2 appears to be relatively unique in its high specificity for and the causal pathway of plaque inflammation. The role of lipoprotein associated phospholipase a 2 lppla 2 enzyme lppla 2 secreted protein 45kda expressed by macrophages, monocytes, t cells and mast cells, circulates in plasma in humans 80% carried by low density lipoprotein ldl molecules catalyses hydrolysis of phospholipids on. Relation of secretory and lipoproteinassociated phospholipase a2 activities to a carotid artery plaque secretoryassociated phospholipase a2 odds ratio or 1. Lipoproteinassociated phospholipase a2 lppla2 also predicts cv disease. Pdf inflammation is thought to play an important role in the. Lipoproteinassociated phospholipase a2 lppla2 is an emerging inflammatory marker that is used to assess the risk for cardiovascular disease cvd and associated events. The role of lppla2 in diabetes mellitus is controversial and analyses, especially in adolescents with type 2 diabetes t2d, are missing. Lipoproteinassociated phospholipase a2 predicts 5year cardiac mortality independently of established risk factors and adds prognostic information in patients with low and medium highsensitivity creactive protein the ludwigshafen risk and. Some recent studies have shown that lppla 2 is an independent risk marker for cardiovascular. Measurement of lipoproteinassociated phospholipase a2 lp pla2 in the assessment of cardiovascular risk 4. Lipoproteinassociated phospholipase a2 and its relationship with markers of subclinical cardiovascular disease.
Lipoproteinassociated phospholipase a 2 lppla 2is an enzyme that has demonstrated proin. Lipoproteinassociated phospholipase a2 lppla2 antigen profile protein summary the protein encoded by this gene is a secreted enzyme that catalyzes the degradation of plateletactivating factor to biologically inactive products. Lipoprotein associated phospholipase a2 lppla2 is a monomeric enzyme that catalyzes oxidized phospholipid found in ldl into lysophosphatidylcholine and oxidized fatty acid, both of which are atherogenic. Several markers of inflammation have shown predictive value for first. Lipoproteinassociated phospholipase a2 activity and mass as. Lipoproteinassociated phospholipase a2, plateletactivating factor acetylhydrolase, generates two bioactive products during the oxidation of low. Measurement of lipoprotein associated phospholipase a2 lp pla2 in the assessment of cardiovascular risk 4. Lipoprotein associated phospholipase a2 and its association with cardiovascular outcomes in patients with acute coronary syndromes in the prove ittimi 22 pravastatin or atorvastatin evaluation and infection therapythrombolysis in myocardial infarction trial. Lipoproteinassociated phospholipase a2 lppla2 appears to. High sensitivity creactive protein hscrp is a marker of metabolic syndrome ms and cardiovascular cv disease. Lipoproteinassociated phospholipase a2 lppla2 elisa kits.
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